IL-21/IL-21R Regulates the Neutrophil-Mediated Pathologic Immune Response during Chlamydial Respiratory Infection

Figure 3

Pulmonary chemokines, cytokines, and TLR/MyD88 signal of IL-21R^-/- mice in response to Chlamydia muridarum (C. muridarum) lung infection. Following C. muridarum infection, mice were euthanized at days 0, 3, 7, and 14 and lungs were harvested for mRNA expression analysis. Total RNA was extracted from pulmonary tissues of wild-type (WT) and IL-21 receptor deficient (IL-21R^-/-) mice, and the mRNA expression of chemotactic factors (a–f) KC, MIP-2, CXCR2, IL-6, IL-1β, and TNF-α as well as (g–j) TLR/MyD88/NF-κB pathway mediators TLR2, TLR4, MyD88, and p65 was measured by quantitative real-time PCR (qPCR). Data are represented as from per group, representative one of three independent experiments. Statistical significances of differences are determined by two-way ANOVA followed by Bonferroni’s multiple comparisons test. , , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)