IL-21/IL-21R Regulates the Neutrophil-Mediated Pathologic Immune Response during Chlamydial Respiratory Infection

Figure 5

Pulmonary chemokines, cytokines, and TLR/MyD88 signal of recombinant murine IL-21- (rIL-21-) administrated wild-type (WT) mice in response to Chlamydia muridarum (C. muridarum) lung infection. The rIL-21 and PBS-treated mice were killed at days 0, 3, 7, and 14 after C. muridarum infection, and total RNA was extracted from the lung tissues to analyze mRNA expression. The quantitative real-time PCR (qPCR) results of chemotactic factors (a–f) KC, MIP-2, CXCR2, IL-6, IL-1β, and TNF-α as well as TLR/MyD88 pathway mediators (g–j) TLR2, TLR4, MyD88, and p65 were shown. Data are represented as from per group, representative one of three independent experiments. Statistical significances of differences are determined by two-way ANOVA followed by Bonferroni’s multiple comparisons test. , , , and .

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