DNMT3a-Mediated Enterocyte Barrier Dysfunction Contributes to Ulcerative Colitis via Facilitating the Interaction of Enterocytes and B Cells

Figure 2

DSS treatment induces intestinal epithelial barrier dysfunction and DNMT3a level. The intestinal epithelial tissues of mice in the sham and DSS group were collected. (a) and (b) ZO-1 and occludin levels in intestinal epithelial tissues were measured by immunofluorescence staining and western blotting. (c) Levels of inflammatory cytokines containing L-10, TGF-β, IL-6 and TNF-α in serum of mice were evaluated by ELISA. (d) The number of CD1d+ B cells and CD138+ B cells in intestinal epithelial tissues was detected by flow cytometry. (e) Total DNA methylation level was measured. (f) The expressions of DNMT1, DNMT3a, and DNMT3b were detected. (g) DNMT3a protein level was determined by immunofluorescence staining assay. , , vs. sham group; .

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