Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-κB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia

Figure 1

Effects of amentoflavone on LPS-induced activation of microglia in BV2 microglial cells. (a) Structure of amentoflavone. (b) Viability of BV2 microglial cells treated with amentoflavone in graded concentrations (0, 0.01, 0.1, 1, 10, 25, 50, 75, and 100 μM) was measured by MTT assay (one-way ANOVA followed by Dunnett’s test). (c) Representative images of immunofluorescence staining of iba-1 in BV2 microglial cells. (d) Semiquantitative analysis of the relative levels of iba-1 by densitometric analysis. (e) Representative immunoblots of iba-1 protein in BV2 microglial cells. (f) Semiquantitative analysis of the relative level of iba-1 by densitometric analysis. Error bars represent . and represent and , respectively. All experiments were performed in triplicate. LPS: lipopolysaccharide; AF: amentoflavone.

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