Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-κB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia

Figure 3

Effects of amentoflavone on LPS-induced inflammatory response in BV2 microglia cells. (a, b) Semiquantitative analysis of the relative levels of IL-1β and TNF-α using ELISA. (c) Semiquantitative analysis of the relative levels of NO. (d) Representative images of immunofluorescence staining of IL-1β in BV2 microglial cells. (e) Semiquantitative analysis of the relative levels of IL-1β in (d) by densitometric analysis. (f) Representative immunoblots of IL-1β, TNF-α, and iNOS protein in BV2 microglial cells. (g) Semiquantitative analysis of the relative level of IL-1β, TNF-α, and iNOS by densitometric analysis. Error bars represent . represent , respectively. All experiments were performed in triplicate. LPS: lipopolysaccharide; AF: amentoflavone.

(a)

(b)

(c)

(d)

(e)

(f)

(g)