MiR-181a-5p Delivered by Adipose-Derived Mesenchymal Stem Cell Exosomes Alleviates Klebsiella pneumonia Infection-Induced Lung Injury by Targeting STAT3 Signaling

Figure 2

Exosomal miR-181a-5p communication between adipose-derived mesenchymal stem cells (ADSCs) and BMDM. (a) The exosomes from ADSCs were observed by TEM. . (b) The expression of CD63 and CD9 was detected by Western blot analysis. (c) The BMDM was treated with exosomes from ADSCs and treated with RNaseA with or without Triton X100. The expression of miR-181a-5p was measured by qPCR. (d) The ADSCs were treated with miR-181a-5p or miR-181a-3p mimic. The expression of miR-181a-3p was analyzed by qPCR in ADSCs or exosomes from ADSCs. (e) The ADSCs were treated with miR-181a-5p or miR-181a-3p and the BMDM was treated with exosomes from the ADSCs. The expression of miR-181a-5p was assessed by qPCR in BMDM. (f) The ADSCs were treated with miR-181a-5p and the BMDM was treated with exosomes from the ADSCs or cotreated with GW4869 (10 M for 48 hours). The miR-181a-5p stained with cyc3-oligo in BMDM was observed by Brightfield and immunofluorescence using confocal analysis. . . Data are presented as . Statistic significant differences were indicated: .

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