MiR-181a-5p Delivered by Adipose-Derived Mesenchymal Stem Cell Exosomes Alleviates Klebsiella pneumonia Infection-Induced Lung Injury by Targeting STAT3 Signaling

Figure 4

MiR-181a-5p is able to target STAT3 signaling. (a) The binding of miR-181a-5p and STAT3 mRNA 3 UTR was predicted in ENCORI website. (b–g) The 293T cells and BMDM were treated with control mimic or miR-181a-5p mimic. (b) The expression of miR-181a-5p was detected by qPCR. (c and d) The luciferase activities of STAT3 mRNA 3 UTR were measured by luciferase reporter gene assays. (e and f) The mRNA expression of STAT3 was analyzed by qPCR assays. (g) The protein expression of STAT3 was tested by Western blot analysis. (h) The protein expression of Nlrp3 and Asc was examined by Western blot analysis. . Data are presented as . Statistic significant differences were indicated: .

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