MiR-181a-5p Delivered by Adipose-Derived Mesenchymal Stem Cell Exosomes Alleviates Klebsiella pneumonia Infection-Induced Lung Injury by Targeting STAT3 Signaling

Figure 6

ADSC-derived exosomal miR-181a-5p attenuates the Klebsiella pneumonia infection-induced lung inflammation. (a–g) The mice received PBS or Klebsiella pneumoniae (10⁴ CFUs). And the mice were treated with exosomes from ADSCs or exosomes from miR-181a-5p-treated ADSCs. (a) The PKH26-labelled miR-181a-5p, CD68, and Ly-6G were detected by immunofluorescence staining. (b) The expression of miR-181a-5p was measured by qPCR in macrophages from BALF. (c) BALF cells and lung sections were analyzed by H&E staining. (d) The numbers of macrophages or neutrophils from BALF were counted. (e) The activity of caspase-1 in murine lung tissue was determined. (f and g) The levels of IL-1β and IL-18 were tested by ELISA in BALF. . Data are presented as . Statistic significant differences were indicated: . (h) ADSC-derived exosomal miR-181a-5p alleviated K. pneu infection-induced lung injury by targeting STAT3 signaling.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)