CpG Oligodeoxynucleotides Inhibit RANKL-Induced Osteoclast Formation by Upregulating A20 Deubiquitinase in RAW 264.7 Cells

Figure 4

CpG-ODN is involved in the regulation of TRAF6, A20, and IL-1β. (a, b) TRAF6 mRNA and protein expression in RAW 264.7 cells under stimulation with either RANKL or CpG-ODN and both. (c) Under the same conditions, TRAF6 protein expression was measured in A20 deficient cells. (d, e) IL-1β mRNA and protein expression were augmented by CpG-ODN in the presence of RANKL. (f) IL-1β protein expression in cells transfected with A20 siRNA under stimulation with either RANKL or CpG-ODN and both. (g, h) A20 expression was measured in cells under stimulation with both RANKL and CpG-ODN by addition of IL-1β (10 ng/ml). Abbreviation: NC: negative control; IL-1β: interleukin-1β. Error bars represent standard deviation of the measurement of three separate samples ( and ).

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