LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling

Figure 3

MDRL attenuates NLRP3 inflammasome and apoptosis in plaques of LDLR^-/- mice fed with HFD. (a) Representative images of IHC staining for NLRP3 in aortic roots from rAAV-CTR- or rAAV-MDRL-treated LDLR^-/- mice. Scale bar: 50 μm. (b) Quantification of NLRP3 positive area in aortic roots. (c and d) Western blots showed the expression levels of NLRP3 protein in aortas from rAAV-CTR- or rAAV-MDRL-treated LDLR^-/- mice. (e) Representative images of TUNEL staining (stained with green) in aortic roots from rAAV-CTR- or rAAV-MDRL-treated LDLR^-/- mice. Scale bar: 50 μm. (f) Quantification of TUNEL-positive cells in aortic roots. (g). Serum concentration of IL-1β from rAAV-CTR- or rAAV-MDRL-treated LDLR^-/- mice. Data are presented as mean ± SEM. , vs rAAV-CTR group.

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