LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling

<div>MDRL regulates NLRP3 inflammasome and apoptosis in VSMCs. (a) The expression of MDRL in MSMCs transfected with siRNA (MDRL-siRNA) or scramble (MDRL-sc) were analyzed by qRT-PCR. (b) The expression of MDRL in MSMCs transfected with adenoviral MDRL (MDRL) or control adenovirus (ctrl) was analyzed by qRT-PCR. (c) Representative images of TUNEL staining in MSMCs transfected with MDRL-sc, MDRL-siRNA, ctrl, or MDRL. Scale bar: 50 <i>μ</i>m. (d) Quantification of TUNEL-positive cells in MSMCs in the indicated groups. (e) Western blots showed the expression levels of NLRP3 in MSMCs in the indicated groups and quantification. The relative expression of NLRP3 was normalized to housekeeping gene <i>β</i>-tubulin in corresponding lane. 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Mediators of Inflammation

fig4

Figure 4

Figure 4: LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling 