Research Article
LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling
Figure 5
MDRL acts as an endogenous sponge to miR-361. (a) The expression levels of miR-361 in MSMCs transfected with siRNA (MDRL-siRNA) or scramble (MDRL-sc) were analyzed by qRT-PCR. (b) The expression levels of miR-361 in MSMCs transfected with adenoviral MDRL (MDRL) or control adenovirus (ctrl) were analyzed by qRT-PCR. (c) Luciferase activities were measured in HEK-293T cells transfected miR-361 sensor luciferase reporter vector, along with MDRL-siRNA or MDRL-sc. (d) Luciferase activities were measured in HEK-293T cells transfected miR-361 sensor luciferase reporter vector, along with ctrl or MDRL. (e) MDRL binds to miR-361. Biotin-conjugated pulldown assay and qRT-PCR were performed in MSMCs. Data are presented as mean ± SEM. vs MDRL-sc or ctrl or miR-361 sensor group.
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