Research Article
LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling
Figure 6
miR-361 binds to SQSTM1 and suppresses SQSTM1/NLRP3. (a) The putative targeting site of SQSTM1 and miR-361 was predicted by Targetscan and PicTar. (b) SQSTM1 and NLRP3 protein expression in MSMCs transfected with miR-361 mimics or control (ctrl). (c) Quantification of SQSTM1 and NLRP3 protein expression on western blots. (d) Luciferase activities were measured in HEK-293T cells transfected with SQSTM1-WT or SQSTM1-MU luciferase reporter vector, along with ctrl or miR-361 mimics. (e) MiR-361 binds to MDRL. Biotin-conjugated pulldown assay and qRT-PCR were performed in MSMCs. (f) Representative images of TUNEL staining in MSMCs transfected with ctrl or miR-361 mimics, and quantification of TUNEL-positive cells. Scale bar: 50 μm. Data are presented as mean ± SEM. vs ctrl group.
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