Topical Treatment of Colquhounia Root Relieves Skin Inflammation and Itch in Imiquimod-Induced Psoriasiform Dermatitis in Mice

Figure 6

CR treatment regulated cell viability and function of M5-induced keratinocytes in vitro. M5 (10 ng/ml) was used to stimulate NHEKs with or without CR treatment ((a): 0, 0.25 μg/ml, 0.5 μg/ml, 1 μg/ml, and 2 μg/ml; (b): 0.5 μg/ml). (a) Cell viability was measured by using CCK-8 (). (b) The mRNA expression of IL-6, CXCL8, IL-1β, CCL20, SP, CGRP, and NGF was detected by RT-qPCR (). The relative mRNA expression was normalized to GAPDH expression. Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s test. values are indicated. .

(a)

(b)