Extracellular CIRP Upregulates Proinflammatory Cytokine Expression via the NF-kappaB and ERK1/2 Signaling Pathways in Psoriatic Keratinocytes

Figure 2

Levels of eCIRP in psoriasis. (a) Detection of eCIRP serum levels in psoriasis patients () and normal controls () by ELISA. (b) eCIRP levels in the sera of IMQ-induced psoriatic mice were measured by ELISA ( mice/group). IMQ: IMQ-induced psoriatic mice; IMQ+C: IMQ-induced psoriatic mice treated with calcipotriol. HaCaT (c) and HEKa (d) cells were stimulated with M5 (10 ng/ml each of IL-1α, IL-17A, IL-22, oncostatin M, and TNF-α) for 24 hours. The eCIRP levels in the culture supernatants were determined using a commercial ELISA kit. Data are expressed as the . , , and vs. control; ^## vs. IMQ.

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