Extracellular CIRP Upregulates Proinflammatory Cytokine Expression via the NF-kappaB and ERK1/2 Signaling Pathways in Psoriatic Keratinocytes

Figure 3

Effects of eCIRP on proinflammatory cytokine expression and the phosphorylation of signaling molecules. (a) rhCIRP caused the time-dependent upregulation of TNF-α, IL-6, and IL-8 in HaCaT and HEKa cells at 30 min and 60 min after 1 μg/ml rhCIRP stimulation; the mRNA levels in the two cell lines were measured by qRT-PCR. (b) HaCaT and HEKa cells were treated with 1 μg/ml rhCIRP for 30 and 60 min, and the phosphorylation of NF-κB p65 and ERK1/2 was analyzed by western blotting. Data are expressed as the . /group. , , and vs. control.

(a)

(b)