mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4⁺ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression

Figure 6

CD4⁺ T cells were assessed for the expression of mTOR pathway-associated proteins, ERS-associated proteins, and apoptosis-associated proteins in WT, LCK-mTOR, LCK-TSC1, CLP, CLP+CIRP-Ab, CLP+NAC, LCK-TSC1+CLP, and Lck-mTOR+CLP mouse groups. Data are . Number of mice per (one-way ANOVA Tukey’s post hoc test). CD4⁺ T cells were purified from CIRP-Ab-treated mice, NAC-treated mice, TSC1 knockout mice, and mTOR mouse spleen lymphocytes. Total proteins were western blotted to identify the expression patterns of mTOR pathway proteins (P-mTOR, mTOR, p70s6k, p-p70s6k, P-4EBP, and 4EBP) (a); CIRP and ERS-associated proteins (GRP78 and CHOP) (b); and apoptosis-related proteins (caspase-3, Bax, and Bcl-2) (c).

(a)

(b)

(c)