Research Article
Magnoliae flos Downregulated Lipopolysaccharide-Induced Inflammatory Responses via NF-κB/ERK-JNK MAPK/STAT3 Pathways
Figure 1
Effect of MF on proinflammatory enzymes and cytokine production in LPS-challenged mice. Mice were intraperitoneally injected with either Dexa, MF, or PBS (as a vehicle) for 1 h before intraperitoneal LPS injection (30 mg/kg) (6 mice per group). (a) Survival rates of these mice were determined 96 h postinjection. (b and c) Liver tissue samples were obtained 4 h post-intraperitoneal LPS injection. Expression of COX-2 and iNOS was determined by western blot with specific antibodies. Densitometric analysis was performed using ImageJ software. β-Actin was used as an internal control. (d and f) Total RNA was isolated from the liver tissue samples, and then, mRNA expression of TNF-α (d) and IL-6 (f) was determined with qRT-PCR. (e and g) Peripheral blood samples were obtained from each mouse. Serum levels of TNF-α (e) and IL-6 (g) were determined using EIA kits. The data are presented as the . ### vs. the control group; , , and vs. the LPS-only-challenged group.
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