Interference of Interleukin-1β Mediated by Lentivirus Promotes Functional Recovery of Spinal Cord Contusion Injury in Rats via the PI3K/AKT1 Signaling Pathway

Figure 3

Expression of IL-1β at the spinal cord. (a) BBB scores in the spinal cords of contused rats on days 1, 3, 5, 7, 14, and 28. BBB scores were significantly lower and increased gradually in the contusion group compared with the sham group. (b) Expression of IL-1β mRNA was detected by qPCR. Data showed that IL-1β mRNA drastically increased and peaked at 6 hours, but there was no obvious change on days 1 and 3 when compared with the sham group. (c) IL-1β was detected by Western blotting, with β-Actin as standard value. Expression of IL-1β increased after spinal cord contusion when compared with the sham group. (d) Immunohistochemistry showed that IL-1β was located in the neurons of the anterior horn of the spinal cord. . IL-1β positive cells were found in the sham group and significantly upregulated at 6 hours and on days 1 and 3. (e) Immunofluorescence double staining for IL-1β/NeuN in neurons of the anterior horn of the spinal cord. DAPI stained blue and IL-1β stained green, NeuN stained red. Coexpression of NeuN with IL-1β within the same cell showed orange (merged). . when compared with the sham group.

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