Interleukin-10-Modified Adipose-Derived Mesenchymal Stem Cells Prevent Hypertrophic Scar Formation via Regulating the Biological Characteristics of Fibroblasts and Inflammation

Figure 2

IL-10-modified ADMSC highly expresses IL-10 and inhibits the proliferation of HSFs. (a) 72 hours after lentivirus infection, we detected the expression of IL-10 mRNA in ADMSC; (b) 72 hours after lentivirus infection, we detected the expression of IL-10 protein in the medium of ADMSC; (c) IL-10 modification reduces the proliferation of ADMSC using CCK8 assay kit; (d) IL-10-modified ADMSC (IL-10-ADMSC) reduced the proliferation of hypertrophic scar fibroblasts (HSFs) in a cocultivation system of ADMSC and HSFs. Data was expressed as (), and each analysis was repeated at least 3 times independently; value was calculated by Student’s test or one-way ANOVA; was , was , and was vs. ADMSC group; ^### was vs. control group.

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