Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells

Figure 1

Poldip2 and Nox4 were upregulated in response to LPS in a dose/time-dependent manner in human lung epithelial cells. (a, b) A549 and Beas-2B cells were treated with the different doses of LPS (0–40 μg/mL for A549 cells, 0–10 μg/mL for Beas-2B cells) for 24 h, and the cell viability was analyzed by MTT assay. (c, d) Confluent cells were treated with different doses of LPS (0–20 μg/mL for A549 cells and 0–5 μg/mL for Beas-2B cells) for 12 h, and the protein levels of Poldip2 and Nox4 were detected by Western blotting and quantitatively analyzed. (e, f) Confluent cells were treated with LPS (10 μg/mL for A549 cells and 1 μg/mL for Beas-2B cells) for the different times (0–24 h), and the protein levels of Poldip2 and Nox4 were detected by Western blotting and quantitatively analyzed. Bars are deviation(SD) of 3 independent experiments. vs. 0 μg/mL or 0 h, vs. 0 μg/mL or 0 h, and vs. 0 μg/mL or 0 h.

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