Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells

<div>Poldip2 interacted with Nox4 induced by LPS with coimmunoprecipitation. (a, b) Confluent cells were treated with LPS (10 <i>μ</i>g/mL for A549 cells and 1 <i>μ</i>g/mL for Beas-2B cells) for 12 h. Cell lysates were immunoprecipitated with normal rabbit IgG (isotype controls) or primary rabbit anti-Poldip2 antibody and immunoblotted with a mouse anti-Nox4 antibody or rabbit anti-Poldip2 antibody. Lysis buffer (Input) is a positive control in coimmunoprecipitation.</div>

Mediators of Inflammation

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Figure 2

Figure 2: Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells 