Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells

Figure 7

Effects of PI3K-AKT signaling on the activation of the Poldip2/Nox4-mediated pathway. Confluent A549 and Beas-2B cells were pretreated with 10 μmol/L LY294002 for 1 h followed by postincubation with LPS (10 μg/mL for A549 cells and 1 μg/mL for Beas-2B cells) for 12 h. (a, b) The protein levels of p-AKT, AKT, Poldip2, Nox4, HO-1, and COX-2 were detected by Western blotting and quantitatively analyzed. (c–h) The expression of Poldip2 and Nox4 in cells was detected by immunofluorescence analysis and a laser confocal microscope. The cells were stained with Poldip2 (green), Nox4 (green), and counter-stained with DAPI (blue) (magnification of 400x). Bars are of 3 independent experiments. vs. control, vs. control, vs. control, vs. LPS, vs. LPS, and vs. LPS.

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