lnc-MRGPRF-6:1 Promotes M1 Polarization of Macrophage and Inflammatory Response through the TLR4-MyD88-MAPK Pathway

Figure 5

lnc-MRGPRF-6:1 may regulate macrophage polarization via the TLR4-MyD88-MAPK pathway. RNA sequencing was performed between lnc-MRGPRF-6:1 knockout macrophage (KO) and macrophage infected by control lentivirus (control). (a) Volcano plot shows differentially expressed genes between KO and control. (b, c) GO and KEGG analysis according to RNA sequencing data. (d) Hierarchical cluster analysis of differentially expressed mRNAs in KO compared to control (partially shown); (e–l) mRNA levels of TLR4, MyD88, P38, JNK, ERK, CXCL10, CXCL11, and IL-1β between KO, control, and after LPS induction were detected by RT-qPCR. (m) Expression of TLR4, MyD88, JNK, ERK, P38, p-P38, p-JNK, and p-ERK between KO and control were analyzed by Western blotting. KO: lnc-MRGPRF-6:1 knockout macrophage; control: macrophage infected by control lentivirus.

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