Nr2f2 Overexpression Aggravates Ferroptosis and Mitochondrial Dysfunction by Regulating the PGC-1α Signaling in Diabetes-Induced Heart Failure Mice

Figure 2

Overexpression of Nr2f2 aggravated cardiac ferroptosis. (a) Representative Western blot of PTGS2 and GPX4 (, , and , ns: not significant, two-way ANOVA). (b) 4-HNE staining (scale ) and mitochondrial transmission electron microscopy (scale ) were identified in the heart tissue. (c–e) Tissue MDA, GSH, and SOD levels in each group (, , and , two-way ANOVA). (f) Total iron level in each group (, , and , two-way ANOVA). (g) Relative gene expression of mitochondrial function and oxidative stress (, , and ; ns: not significant, two-way ANOVA).

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