Nr2f2 Overexpression Aggravates Ferroptosis and Mitochondrial Dysfunction by Regulating the PGC-1α Signaling in Diabetes-Induced Heart Failure Mice

Figure 3

Knockdown of Nr2f2 alleviated ferroptosis induced by palmitic acid in neonatal rat cardiomyocytes. (a) Representative Western blot of PTGS2 and GPX4 (, , and ; ns: not significant, two-way ANOVA). (b) MDA level in each group (, , ; ns: not significant, two-way ANOVA). (c) Lipid ROS was assessed by C11 BODIPY 581/591 staining coupled with flow cytometry analysis (, , and ; ns: not significant, two-way ANOVA). (d) Cell viability was measured by CCK8 (, , , two-way ANOVA). (e) Mitochondrial membrane potential was measured by JC-1 staining; red fluorescence represents healthy mitochondria, while green indicates loss of mitochondrial potential (scale , , , , two-way ANOVA). (f) Intracellular ATP level in each group (, , , , and ; ns: not significant, two-way ANOVA). (g) Relative expression of mitochondrial energy metabolic genes (, , NC-BSA vs. siNr2f2-BSA, and ; NC-PA vs. siNr2f2-PA, , two-way ANOVA).

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