Nr2f2 Overexpression Aggravates Ferroptosis and Mitochondrial Dysfunction by Regulating the PGC-1α Signaling in Diabetes-Induced Heart Failure Mice

Figure 4

Nr2f2 regulates ferroptosis and mitochondrial dysfunction via PGC-1α. (a) Representative Western blot of the PGC-1α protein level after Nr2f2 overexpression and knockdown (, , and , Student’ -test). (b) MDA level in each group (, , ; ns: not significant, two-way ANOVA). (c) Representative Western blot of PTGS2 and GPX4. (, , and , two-way ANOVA). (d) Lipid ROS was assessed by C11 BODIPY 581/591 staining coupled with flow cytometry analysis (, , , two-way ANOVA). (e) Cell viability was measured by CCK8 (, , , two-way ANOVA). (f) Representative JC-1 staining (scale , , ; ns: not significant, , two-way ANOVA). (g) Intracellular ATP level in each group (, , , two-way ANOVA).

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