Limb-Bud and Heart (LBH) Upregulation in Cardiomyocytes under Hypoxia Promotes the Activation of Cardiac Fibroblasts via Exosome Secretion

Figure 4

The effects of the internalization of CM^LBH+ exosomes on the proliferation, differentiation and migration of CFs. (a) Protein levels of LBH, CRYAB, collagen I, α-SMA, E-cadherin, and vimentin in mouse CFs treated with hypoxic CM-derived exosomes and in rat CFs treated with H9c2-LBH-derived exosomes ( and vs. 21%-Exo/Lv5NC-Exo treated). (b) Representative immunofluorescence images of α-SMA and collagen I expression in mouse CFs treated with hypoxic CM-derived exosomes and in rat CFs treated with H9c2-LBH-derived exosomes. (c) EdU staining of mouse CFs treated with hypoxic CM-derived exosomes and rat CFs treated with H9c2-LBH-derived exosomes ( and vs. 21%-Exo/Lv5NC-Exo treated). (d) Representative images of the Transwell assay of mouse CFs treated with hypoxic CM-derived exosomes and rat CFs treated with H9c2-LBH-derived exosomes and the corresponding statistical analysis ( vs. 21%-Exo/Lv5NC-Exo treated).