CD4⁺ T Cells Promote IgG Production in MHC-Independent and ICAM-1-Dependent Manners in Pristane-Induced Lupus Mice

Figure 5

ICAM-1 attenuates IgG production in the coculture of CD4⁺ T cell and B cell interaction from PBS or pristane-treated mice. CD4⁺ T cells and B cells were sorted from PBS and pristane-treated BALB/c mouse (6 months later) by MACS, respectively. CD4⁺ T cells were cultured with B cells derived from PBS or pristane mice (on a BALB/c background). (a) Flow cytometric analysis of ICAM-1 expression on CD4⁺ T cells. (b) Increased expression levels of ICAM-1 on CD4⁺ T cells from lupus-like mice. (c–f) Total IgG (c), IgM (d), IgG1 (e), and IgG2a (f) levels in the supernatants of different coculture groups (T+B, T+B+ISO, and T+B+anti-ICAM-1) were quantified by the ELISA assays. Each group had at least five mice for the analysis. Data are represented by mean of OD₄₅₀ values with bar of SEM from five mice. ; ; ; .

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