Hyperoside Attenuates Sepsis-Induced Acute Lung Injury (ALI) through Autophagy Regulation and Inflammation Suppression

Figure 3

HYP treatment attenuated LPS-induced HLMVEC damage by regulating Atg13-mediated autophagy. (a and b) RT-qPCR detection showcased the expression of Atg13 and LC3 in HLMVEC cells. (c–e) ELISA detection showed the expression of inflammatory factors TNF-α, IL-1β, and IL-6. (f and g) HLMVEC cells apoptosis was assayed via flow cytometry after Annexin V–FITC staining. (h and i) In vitro tube formation of HLMVECs. The total branching point was analyzed. Data are denoted as means ± SD. , , vs. the NC group. ^#, ^##, ^### vs. the LPS group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)