BCL-3 Promotes Intracerebral Hemorrhage Progression by Increasing Blood–Brain Barrier Permeability, Inflammation, and Cell Apoptosis via Endoplasmic Reticulum Stress

Figure 4

ERS activator reversed the role of BCL-3 siRNA on BBB permeability and inflammation and terminated cell apoptosis in BMECs. (a) The expression level of BCL-3 was tested by western blotting. (b) The expression level of occludin and claudin-5 was evaluated by qPCR. (c) ELISA measured the levels of inflammatory factors of IL-6, IL-1β, and TNF-α. (d, e) TUNEL and FCM were applied to evaluate cell apoptosis. (f) Western blotting performed on ERS-related proteins, including p-IRE1α, GRP78, and CHOP. (f) TEM was performed to observe ER. .

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