Salmonella Typhimurium Infection Reduces the Ascorbic Acid Uptake in the Intestine

Figure 4

Effect of IL-1β on AA uptake, hSVCT1 expression levels, and the activity of SLC23A1 promoter in Caco-2 cells. Concentration-dependent effect of IL-1β treatment on the hSVCT1 mRNA expression (a). Determined AA uptake in IL-1β (50 ng/ml)-exposed and control cells (b). Performed western blotting to quantify the expression of hSVCT1 protein in IL-1β-exposed and control cells (c). The activity of SLC23A1 promoter was determined in IL-1β-treated and control cells (d). Values are of at least 3-4 independent experimental runs using different passage of cells. , , and .

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