LIGHT Amplification by NF-κB Contributes to TLR3 Signaling Pathway-Induced Acute Hepatitis

Figure 3

Blockade of LIGHT with recombinant HVEM-Fc fusion protein ameliorates liver injury induced with poly(I:C). (a) BALB/c mice were pretreated with recombinant HVEM-Fc protein (100 μg/mouse) one hour before a lethal dose of poly(I:C) (2.5 μg/mouse) and D-GalN (10 mg/mouse) injection () or poly(I:C) and D-GalN () only. The survival of mice was monitored. (b) BALB/c mice were pretreated with recombinant HVEM-Fc protein (100 μg/mouse) one hour before a sublethal dose of poly(I:C) and D-GalN injection () or poly(I:C) and D-GalN () only. At 8, 16, and 24 h time point, serum ALT levels were measured and data are shown as ; , . Mice were sacrificed at 24 h and the liver tissues were fixed and stained with H&E (size bar represents 50 μm) (c) and TUNEL staining (size bar represents 20 μm) (d).

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