LIGHT Amplification by NF-κB Contributes to TLR3 Signaling Pathway-Induced Acute Hepatitis

Figure 4

TLR3-induced LIGHT expression through activation of NF-κB. (a) BALB/c mice were injected with poly(I:C) (1.5 μg/mouse) and D-GalN(10 mg/mouse). Mice were pretreated with NF-κB inhibitor (BAY-11-7082) one hour before poly(I:C) injection. The mice were sacrificed and total RNA was extracted from the livers. Expression of LIGHT mRNA was measured by real-time PCR at the indicated time points, and data are shown as ; , . (b) Expression LIGHT protein was analyzed by western blot at 12 h after poly(I:C) injection. (c) Mice were sacrificed at 24 h, the liver tissues were fixed and stained with H&E (size bar represents 20 μm).

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