Curcumin Improves Diabetic Cardiomyopathy by Inhibiting Pyroptosis through AKT/Nrf2/ARE Pathway

Figure 4

Curcumin promotes the translocation of Nrf2 into the nucleus through the AKT pathway. Glu: glucose; DM: diabetic rat; Cur: curcumin; the dose of curcumin used in cell experiments was 14 μM; the dose used in the rat experiment was 200 mg/kg/day. (a) AKT/Nrf2/ARE pathway protein expression levels in cardiomyocytes were detected using western blotting. (b) The expression of the Nrf2 protein in the nucleus (nu-Nrf2) of cardiomyocytes was detected by western blotting. (c) Expression levels of immunofluorescence-labeled Nrf2 in different parts of cells (20x). (d) The expression sites of the Nrf2, DAPI, and KEAP1 proteins were analyzed for fluorescence colocalization using ImageJ. (e) Western blotting detection of Nrf2 protein expression in the nucleus of rat myocardial tissue. (f) Western blotting was performed to detect the expression levels of proteins in the AKT/Nrf2/ARE pathway in rat myocardial tissue. : high-glucose group vs. normal medium and curcumin treatment group. #: curcumin-treated group vs. curcumin-treated group. ^^: diabetes group vs. control group and curcumin-treated group. ^: curcumin-fed vs. no curcumin (in diabetic rats). Values are expressed as the deviation.

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