Research Article
Curcumin Improves Diabetic Cardiomyopathy by Inhibiting Pyroptosis through AKT/Nrf2/ARE Pathway
Figure 7
Curcumin ameliorated nigericin-induced pyroptosis. Nig: nigericin; the concentration used in the experiment was 10 μM. Cur: curcumin; the dose of curcumin used in cell experiments is 14 μM. Si-Nrf2: interference virus vector targeting the Nrf2 gene. (a) Western blotting to detect the concentration of pyroptosis-related proteins. (b) MitoTracker detects the relative expression of mitochondrial reactive oxygen species (ROS). The stronger the red, the more obvious the accumulation of ROS in the mitochondria; the mitochondrial tracer is shown in green (20x). (c) The CCK-8 test detected cell activity in each group. : nigericin-added group vs. control group and curcumin-treated group. #: Si-Nrf2 transfected group vs. Si-Nrf2 nontransfected group (in nigericin-supplemented medium). Values are expressed as the deviation.
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