Identifying Macrophage-Related Genes in Ulcerative Colitis Using Weighted Coexpression Network Analysis and Machine Learning

Figure 4

Validation of ARGs at single-cell resolution and stratification of integrated UC cohorts. Dot plot (a) of cell type marker genes and UMAP (b) of major immune cell clusters. (c) UMAP of MRGs signature enriched in major cell clusters. (d) Heat map depicting consensus clustering solution (k = 3) for macrophage-related genes in UC samples (e) Unsupervised clustering of the 52 ARGs divided the UC patients in the integrated cohort into three groups. (f) The main 10 pathways in the three subtypes were significantly activated (red) or inhibited (green) according to fGSEA. (g) Violin plots (box plots) of macrophage infiltration levels and inflammation scores for the three subtypes.

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