Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor

Figure 11

Reporter activities of wildtype and mutant CXCL2 promoters in HEK293T cells. HEK293T cells were transfected with luciferase reporter plasmids for (a) wildtype, (b) 407 bp deletion, (c) HNF4α mutant, (d) NF-κB mutant, (e) CRE mutant, and (f) NF-κB/CRE dual mutant CXCL2 promoters, dexamethasone groups transfected with GR expression plasmid, HNF4A groups transfected with HNF4α1 expression plasmid, and incubated overnight. The following day cells were treated with dexamethasone 10 nM, TNFα 20 ng/mL, and/or DMSO control for 24 hr; and then promoter activities were quantified by dual-luciferase assay. Mean ± SE, n = 3–4 replicate wells per sample, and , , and . ns, not significantly different.

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