Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor

Figure 9

Basal reporter activities of wildtype and mutant CXCL2 promoters in HEPG2 and HEK293t cell lines. (a) Diagram of promoter (red bar) regions that were mutated or deleted, and data mining of ChIP-seq (GSE22078) of binding of HNF4α as well as histone marks trimethylation of lysine-4 of histone H3 (H3K4me3, GSM2533942) and monomethylation lysine-4 of histone H3 (H3K4me1, GSM2700191). The ChIP-seq data were retrieved from GEO DataSets and visualized in Integrative Genomics Viewer (IGV) [29, 30]. Promoter activities in (b) HEPG2 cells and (c) HEK293T cells were quantified by dual-luciferase assay after the transfected cells were treated with DMSO (0.1%) for 24 hr. (HEPG2 data is from the same experiment as Figure 10, graphed relative to WT plasmid control as opposed to DMSO.) Mean ± SE, n = 4 replicate wells per promoter. , , and . ns, not significantly different.

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