Mechanism of SET8 Activates the Nrf2-KEAP1-ARE Signaling Pathway to Promote the Recovery of Motor Function after Spinal Cord Injury

Figure 1

si-Keap1 alleviates LPS-induced oxidative damage induction and inhibits astrocyte autophagy. (a) Protein expression of Keap1 in astrocytes under LPS conditions under normal, LPS, and Keap1 knockdown conditions. (b) Expression of the associated autophagy proteins LC3II/I, P62, and Beclin1 in astrocytes under NC, LPS and LPS+Keap1 conditions. (c) Expression of the signature proteins GFAP and Vimentin of astrocytes under NC, LPS, and LPS+Keap1 conditions. (d) Expression of brevican and neurocan, a protein associated with glial scar formation, in NC, LPS, and LPS+Keap1 conditions. (e) DHE staining to detect the level of ROS in astrocytes under NC, LPS, and LPS+Keap1 conditions.

(a)

(b)

(c)

(d)

(e)