Mechanism of SET8 Activates the Nrf2-KEAP1-ARE Signaling Pathway to Promote the Recovery of Motor Function after Spinal Cord Injury

Figure 5

SET8 promotes motor function recovery after SCI in vivo in rats. (a) Analysis of SET8 and Keap1 expression in the sham, model, and OE-SET8 groups by immunohistochemistry; Western blotting of the astrocyte autophagy-related proteins LC3II/I, P62, and Beclin1 in (b), the glial scar formation-related proteins brevican and neurocan in the sham, model, and OE-SET8 groups in (c), and OE-SET8 expression in the different groups. (d) Immunofluorescence analysis of astrocyte morphology and glial scar formation in the sham, model, and OE-SET8 groups. The number of surviving neurons in the sham, model, and OE-SET8 groups was detected by Nissl staining. (e) The number of surviving neurons was detected by Nissl staining in the sham, model, and OE-SET8 groups.

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