RhoA Promotes Synovial Proliferation and Bone Erosion in Rheumatoid Arthritis through Wnt/PCP Pathway

Figure 2

Impact of Sh-RhoA on migration, apoptosis, viability, and inflammatory response of RA-FLSs. (a) The effects of Sh-RhoA infection on protein expression. (b) Differences in the relative ratios of RhoA to GAPDH between three groups (n = 3); , Sh-RhoA versus blank; , Sh-RhoA versus Sh-Ctr. (c and d) The results of wound healing assay showed that the cell migration ability in the Sh-RhoA group was lower than that in the control group, . (e) The results of Transwell cell migration test in RA-FLSs. (f) The number of migration cells in Sh-RhoA group was lower than that in the Sh-Ctr group, . (g) Representative images showing the Tunel + cells (red) of Sh-RhoA group and control. The nucleus was stained with Hoechst solution (blue). (h) The statistic percentage of Tunel + cells in indicated conditions, . (i) The results of CCK-8 assay revealed that Sh-RhoA reduced the viability of RA-FLSs, , 0.004. (j and k) Western blot analyses revealed that in the Sh-RhoA group, the expression of MMP-3 and MMP-13 was lower than that in the control group, , 0.003. (l) The relative mRNA levels of TNF-α, IL-1β, IL-17, and IL-21. Sh-RhoA only restrained the secretion of IL-17 (, 0.088, 0.001, 0.120). (m) The relative mRNA levels of OPG and RANKL. Sh-RhoA inhibited the level of RANKL in RA-FLSs (, 0.006). (n) The ratio of OPG/RANKL increased significantly in the Sh-RhoA group (n = 3), . , , .

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