RhoA Promotes Synovial Proliferation and Bone Erosion in Rheumatoid Arthritis through Wnt/PCP Pathway

Figure 3

Expression of RhoA in OC differentiation and the effect of Sh-RhoA on OC formation. (a–c) Differences in the relative protein and mRNA levels of RhoA between three groups (n = 3); the relative protein (, 3 days vs. 0 day) (a and b) and mRNA (, 3 days vs. 0 day) (c) levels of RhoA enhanced obviously on the third day. (d) The typical fluorescence images of transfected BMMCs differentiated into OCs. (e) The level of RhoA mRNA was lower in BMMCs transfected with Sh-RhoA lentivirus, . (f) Differences in the percentage of mature OCs between three groups; the percentage of mature OCs in the Sh-RhoA group was lower than that in the Sh-Ctr group, . (g) Differences in the BMMC membrane fusion rate between three groups; the BMMC membrane fusion rate was significantly reduced in the Sh-RhoA group compared with the Sh-Ctr group, . (h) The results of TRAP assay. (i) Dil fluorescence staining of cell membrane during BMMC fusion (n = 3). , , .

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