Low-Dose Taxol Promotes Neuronal Axons Extension and Functional Recovery after Spinal Cord Injury

Figure 3

Low-dose Taxol promotes neuronal differentiation from BNSCs and extension in vitro. (a) Low-dose Taxol significantly increases the number of Tuj1-positive cells; p <0.05, p <0.01, p <0.001. (b) Low-dose Taxol significantly extends the average axonal length of neurons, p <0.05, p <0.01, p <0.001. (c) Effects of low-dose Taxol on BNSC differentiation in vitro; Green, Tuj1; Red, Map2; Blue, DAPI. Scale: 100 μm. (d) During BNSCs differentiation, the addition of Taxol at a low dose increases the number of Tuj1 and Map2 positive co-stained cells. As compared to the no-Taxol group, Tuj1 and Map2 co-stained positive cells in the Taxol 3.5 nmol/L and 5 nmol/L groups are higher, p <0.001, and in Taxol 7 nmol/L group, p <0.05. (e) The filament-like neurites of GFAP-positive glial cells derived from BNSCs are affected by the concentration of Taxol in the medium. With the increase in Taxol concentration, the filament-like neurites gradually elongate, presenting the characteristics of a “track-like” parallel arrangement.

(a)

(b)

(c)

(d)

(e)