Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease

<div>VTN suppressed intestinal epithelial cell differentiation. (a) Immunofluorescence of VTN expression level in indicated intestinal tissue from clinical sample and colitis model. (b) Real-time PCR and (c) Western blotting and quantified analysis were performed to detect the effect of VTN on CDX2 and MUC2 expression. Data presented as the mean ± s.e.m. of three independent experiments and were analyzed by <i>t</i> test, <span class="nowrap"><svg height="13.532pt" id="M1" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 82.0539 13.532" width="82.0539pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,2.179,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 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42 199 34 109 27V0H384Z"></path></g></svg>.</span> (d) Intestinal organoid assay was used to explore the effect of VTN on intestinal organoid generation. (e) The immunofluorescence was performed to analyze CDX2 and MUC2 expression in intestinal organoid in response to VTN stimulation described as (d).</div>

Mediators of Inflammation

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Figure 1

Figure 1: Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease 

Figure 1 | Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease 