Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease

Figure 5

Inhibition of PDE4 alleviated colitis. In a DSS-induced acute colitis model, mice were treated with dipyridamole (10 mg/kg) daily for 1 month, and the body weight was recorded every 2 days (a) The statistical difference was performed by one-way ANOVA, , control, n = 6; DSS + vehicle, n = 6; DSS + dipyridamole, n = 6, data presented as the mean ± s.e.m. (b) The representative image of colon length was captured and the statical analysis were performed by one-way ANOVA, , data are represented as mean ± s.e.m.; . (c) Immunofluorescence of indicated protein expression level was used to show the effect of DIP in DSS-induced colitis, quantitation was performed by Image J, and data presented as the mean ± s.e.m and analyzed by one-way ANOVA, , MFI: mean fluorescence intensity. (d) Schematic view of IBD patients enrolled in a pilot clinical study received endoscopy assessment and biopsy collection. The information for the pilot study is provided in Table 1. (e) Analysis of clinical disease activity index (DAI) evaluation in five IBD children in indicated timepoint. Data are represented as mean ± s.e.m; p values were calculated by t test. n = 5, . (f) The representative endoscopic images of before and after DIP administration in five children with IBD, and score for endoscopy was evaluated. (g) Immunofluorescence of indicated protein expression level was used to show the effect of DIP in patients with IBD, quantitation was performed by Image J, and data presented as the mean ± s.e.m and analyzed by paired t test, , MFI: mean fluorescence intensity. (h) Schematic model of VTN-regulated intestinal epithelial cell differentiation through ferroptosis.

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