Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus Kidney

Figure 4

Inhibition of SLC7A11 enhanced ferroptosis of kidney B cells in lupus mice. (a) Flow cytometry analysis showed Sulfasalazine treatment (inhibiting xCT) upregulated B cell ferroptosis in lupus mice. Dead cells were removed by counterstaining fixable viability dye. (b) Dot plot showed statistical analysis of the ratio of FerronOrange+ B cell/total B cells in lupus kidney, either treated with PBS (), or with sulfasalazine (). Data represents similar results from at least 2 independent experiments. . (c) Dot plot showed statistical analysis of the absolute cell numbers of FerronOrange+ B cell in lupus kidney, either treated with PBS (), or with sulfasalazine (). Data represents similar results from at least 2 independent experiments. . (d) Flow cytometry analysis showed inhibited B cell proliferation in lupus kidney, after sulfasalazine treatment. Dead cells were removed by counterstaining fixable viability dye. (e) Dot plots showed statistical analysis of the proliferating B cells frequencies(Ki67+ B cells) and numbers in lupus kidney, either treated with PBS (), or with sulfasalazine (). Data represents similar results from at least 2 independent experiments. .

(a)

(b)

(c)

(d)

(e)