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Journal of Neural Transplantation and Plasticity
Volume 3 (1992), Issue 2-3, Pages 139-150
http://dx.doi.org/10.1155/NP.1992.139

Transplantation of Human Neuroblastoma Cells, Catecholaminergic and Non-Catecholaminergic: Effects on Rotational Behavoir in Parkinson's Rat Model

1Department of Neurology, University of California at Los Angeles School of Medicine, Los Angeles, California 90024-1769, USA
2Department of Surgery, Division of Neurosurgery, University of California School of Medicine, Los Angeles, CA 90024, USA
3Department of Surgery, Cedars Sinai Medical Center, Los Angeles, CA 90048-1869, USA
4Department of Hematology/Oncology, USC-Children's Hospital of Los Angeles, Los Angeles, CA 90054-0700, USA

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cultured human catecholaminergic and noncatecholaminergic donor cells were used in neural transplantation experiments in a rat model of Parkinson's disease. Using two different human catecholaminergic neuroblastoma cell lines, one control non-catecholaminergic neuroblastoma cell line, and one sham control (tissue culture medium), transplants were made into the striatum using a modified Ungerstedt hemiparkinsonian rat model. Significant decreases in apomorphine-induced rotational behavior were produced by two of three catecholaminergic cell lines. Grafted cells staining positively for tyrosine hydroxylase (TH) and catecholamine fluorescence indicated viable catecholamine activity in the two cell lines which produced reductions in rotational behavior. Catecholamine fluorescence was not detected in either of the two controls. These data suggest a link between catecholamine secretion by transplanted cells and motor improvement using a rat rotational behavior model.