Spontaneous Plasticity of Multineuronal Activity Patterns in Activated Hippocampal Networks
Figure 1
Physiologically relevant ionic conditions induce a reversible increase in spontaneous activity of hippocampal
CA3 neurons in organotypic cultures. (a) Simultaneous loose patch-clamp recording
and time-lapse imaging of somatic calcium signal from a hippocampal CA3 neuron
loaded with Oregon green 488 BAPTA-1AM. The timings of action potentials can be
reconstructed from the onset timings of individual calcium rise events. (b)
Representative example of a change in CA3 network activity following 15-min replacement
of normal extracellular solution with pACSF. Each dot represents a single-calcium transient.
(c) pACSF-induced change in the percentage of active cells to the total number
of cells monitored. (d) pACSF-induced change in the mean event frequency per cell.
Open and closed circles indicate group without ( slices, Control) and with pACSF replacement ( slices, pACSF), respectively. Data
are means ± SEMs.
