Directed Fiber Outgrowth from Transplanted Embryonic Cortex-Derived Neurospheres in the Adult Mouse Brain
Figure 1
Analysis of neurosphere growth and differentiation in vitro. (a) Low power view (bright field) of a neurosphere culture. Many cortex-derived neurospheres had developed after 7 days in vitro. Scale bar = 50 m. (b) Neurospheres expressing a Tau-GFP fusion protein from the cortex of Tau-GFP transgenic mice. (c) DAPI staining of a neurosphere culture. (d) Within the cultured neurospheres, NG2 positive cells (red) were typically located in the center of the rosettes. (e) Tau-GFP expression was present throughout the neurospheres. (f) Merged image between DAPI, Tau-GFP, and NG2 showing the central location of NG2 positive cells within the neurosphere. Scale bar = 20 m. (g) One week after transfer to poly-D-lysine-coated dishes, Tau-GFP-positive neurosphere-derived cells had attached and extended processes. Scale bar = 20 m. (h) Network of cellular processes from neurosphere-derived cells as seen with Tau-GFP expression. (i) Staining with the neuronal marker -III-tubulin (H) confirms the neuronal differentiation of many neurosphere-derived cells. Scale bar = 50 m.
